As a cornerstone of the body’s immune response, antibodies can provide significant data to support scientists’ research. Antibodies are used in a multitude of applications in research, including but not limited to western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), chromatin immunoprecipitation (ChIP), and flow cytometry (FC).
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If you would like to learn more about the different types of antibodies, their differences (i.e. rabbit versus mouse), and their uses in research, let's take a look below:
Antibodies (also known as immunoglobulins) are protective proteins produced by the immune system in response to antigens. It is a Y-shaped molecule consisting of two heavy and two light chains held together by disulfide bonds.
Each crest of the Y-shaped structure contains a paratope that can specifically bind the epitope to the appropriate antigen. Monoclonal and polyclonal are the two main types of antibodies.
Monoclonal antibodies (mAbs) recognize antigens and bind to epitopes, whereas polyclonal antibodies (pAbs) recognize antigens but bind to many different epitopes. The mAbs and pAbs production processes are different and offer significant support for both research and diagnostic purposes.